Combating Drug Resistant Pathogenic Bacteria Isolated from Clinical Infections, with Silver Oxide Nanoparticles
نویسندگان
چکیده
The antibiogram study of methicillin resistant Staphylococcus aureus isolates revealed 100% resistance to vancomycin, bacitracin, erythromycin, ciprofloxacin and nalidixic acid. Eight isolates (53.3%) showed resistance to co-trimoxazole and one isolate to rifampicin, which was the drug of choice. An effort was made to evaluate the antimethicillin resistant Staphylococcus aureus activity of silver oxide (Ag2O) nanoparticles synthesized from Aspergillus terreus VIT 2013. Production of Ag2O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm. X-ray diffraction pattern showed 2θ values at 27, 32, 38 and 57°, which corresponded to the cubic structure of Ag2O nanocrystals. Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO2 and silver, revealing protein mediated nanoparticle production. The scanning electron microscope image showed freely dispersed Ag2O nanoparticles. The nanoparticles were active against all methicillin resistant isolates and hence can be used as antibacterial agents against drug resistant bacteria.
منابع مشابه
The effect of antibacterial synergism of silver nanoparticles with extract of Urtica dioica and Allium hirtifolum against multidrug resistant klebsiella (MDR) isolated from ICU patients
Background: Because most bacteria responsible for hospital infections are resistant to common antibiotics, the tendency to produce effective and nontoxic anti-microbial drugs have increased. Therefore, it is attempted to substitute antibiotics with natural products, such as nanoparticles and plant extracts. The aim of this study was to investigate the synergistic antibacterial effects of silver...
متن کاملEvaluation of the Effect of Zinc Oxide Nanoparticles on the Inhibition of Biofilm formation of standard Pathogenic Bacteria and Comparison with Drug Resistant Isolates
Introduction: Traditional medicines cannot adequately reach the target tissues, due to their large size; therefore, the attention of researchers has been drawn to the use of nanomedicines. In fact, the use of biological active compounds loaded on the surface of nanoparticles can be effective the in the promotion of their antimicrobial activity. In the earlier studies, it was demonstrated that b...
متن کاملComparison of Inhibitory Effects of Silver and Zinc Oxide Nanoparticles on the Growth of Plant Pathogenic Bacteria
In this research, the inhibitory effects of silver nanoparticles and zinc oxide nanoparticles, in vitro, on disease stone fruits bacterial canker caused by Pseudomonas syrigae pv. Syringae and disease bacterial blight caused by Xanthomonas arboricola pv. juglandis, were studied. Different concentrations of nanoparticles were prepared on Mueller Hinton agar medium in two different ways in a com...
متن کاملA Study on the Antimicrobial Effect of Zinc Oxide Nanoparticles on Clinical Strains of Staphylococcus aureus Resistant to Vancomycin
Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics.Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria.The aim of this study isto investigate the preval...
متن کاملBactericidal effect of silver nanoparticles against multidrug-resistant bacteria
Infections caused by drug-resistant microorganisms result in significant increases in mortality, morbidity, and cost related to prolonged treatments. The antibacterial activity of silver nanoparticles against some drug-resistant bacteria has been established, but further investigation is needed to determine whether these particles could be an option for the treatment and prevention of drugresis...
متن کامل